Regulation of cell wall synthesis and assembly in grasses

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Title
Regulation of cell wall synthesis and assembly in grasses

CoPED ID
ce27bb20-fce4-4b44-a9f3-b3a288f9cb00

Status
Closed

Funder

Value
£416,000

Start Date
March 31, 2008

End Date
March 30, 2012

Description

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The composition and cross-linking of cell wall polymers determines the efficiency by which biomass can be processed to release the abundant cell wall sugars for biofuel production. A better understanding of the cell wall structure is therefore essential to optimise the energy-potential of grasses and to enable the engineering and breeding of varieties in which cell-wall composition and cross-linking is optimized for conversion. It is estimated that more than a thousand genes are involved in the synthesis and remodelling of cell walls, but only a handful of genes have been characterised. Despite the considerable interest in developing grasses as a renewable energy source, the discovery of genes involved in cell wall biogenesis in these monocots is particularly poor. To optimize the amount, composition, and structure of cell walls in grasses we are identifying some of the genes involved. Due to the available genetic tools, its close phylogenetic relationship with energy grasses including Miscanthus, and C4 photosynthesis, maize is an ideal model for the discovery of cell wall related genes and the translation of gene-function discovery to more genetically recalcitrant bioenergy crops such as Miscanthus. Differential gene expression profiles have been determined by comparing elongating and non-elongating maize internodes using maize microarrays. Key candidate genes predicted to fulfil crucial roles in cell wall biosynthesis and remodelling will be targeted for functional testing in models with the aim that results be translated into Miscanthus. New tools including the production of antibodies that specifically recognise ferulic acid dimers are also being developed (in collaboration with Prof. Paul Knox, University of Leeds and Prof. John Ralph, University of Wisconsin-Madison) as these can be used to study the temporal and spatial aspects of cell wall cross-linking mediated by ferulic acid dimers.

Iain Donnison PI_PER
Gordon Allison COI_PER
Maurice Bosch COI_PER

Subjects by relevance
  1. Genes
  2. Cell walls
  3. Cells
  4. Maize
  5. Bioenergy
  6. Energy crops
  7. Cell biology
  8. Renewable energy sources

Extracted key phrases
  1. Cell wall synthesis
  2. Abundant cell wall sugar
  3. Cell wall structure
  4. Cell wall polymer
  5. Cell wall biogenesis
  6. Wall composition
  7. Energy grass
  8. Differential gene expression profile
  9. Key candidate gene
  10. Regulation
  11. Renewable energy source
  12. Function discovery
  13. Ferulic acid dimer
  14. Prof. John Ralph
  15. Prof. Paul Knox

Related Pages

UKRI project entry

UK Project Locations