History of changes to: Engineering more water-use efficient crops: functional genomics of weak and strong Crassulacean acid metabolism in diverse species of the genus Kalanc
Date Action Change(s) User
Nov. 27, 2023, 2:13 p.m. Added 35 {"external_links": []}
Nov. 20, 2023, 2:03 p.m. Added 35 {"external_links": []}
Nov. 13, 2023, 1:34 p.m. Added 35 {"external_links": []}
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July 31, 2023, 1:34 p.m. Added 35 {"external_links": []}
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July 10, 2023, 1:26 p.m. Added 35 {"external_links": []}
July 3, 2023, 1:26 p.m. Added 35 {"external_links": []}
June 26, 2023, 1:26 p.m. Added 35 {"external_links": []}
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April 3, 2023, 1:26 p.m. Added 35 {"external_links": []}
Jan. 28, 2023, 11:08 a.m. Created 43 [{"model": "core.projectfund", "pk": 28621, "fields": {"project": 5832, "organisation": 7, "amount": 0, "start_date": "2017-09-30", "end_date": "2022-03-30", "raw_data": 46097}}]
Jan. 28, 2023, 10:52 a.m. Updated 35 {"status": ["Active", "Closed"]}
Jan. 28, 2023, 10:52 a.m. Added 35 {"external_links": []}
April 11, 2022, 3:47 a.m. Created 43 [{"model": "core.projectfund", "pk": 20747, "fields": {"project": 5832, "organisation": 7, "amount": 0, "start_date": "2017-09-30", "end_date": "2022-03-30", "raw_data": 27329}}]
April 11, 2022, 3:47 a.m. Created 41 [{"model": "core.projectorganisation", "pk": 78614, "fields": {"project": 5832, "organisation": 1886, "role": "LEAD_ORG"}}]
April 11, 2022, 3:47 a.m. Created 40 [{"model": "core.projectperson", "pk": 48436, "fields": {"project": 5832, "person": 1648, "role": "STUDENT_PER"}}]
April 11, 2022, 3:47 a.m. Created 40 [{"model": "core.projectperson", "pk": 48435, "fields": {"project": 5832, "person": 8317, "role": "SUPER_PER"}}]
April 11, 2022, 1:48 a.m. Updated 35 {"title": ["", "Engineering more water-use efficient crops: functional genomics of weak and strong Crassulacean acid metabolism in diverse species of the genus Kalanc"], "description": ["", "\nCrassulacean acid metabolism (CAM) is an adaptation of photosynthesis found in a diverse range of plant species that inhabit arid and semi-arid environments. CAM plants can achieve water use efficiencies up to ten-times greater than C3 species. Our over-arching goal is to develop a systems level view of CAM by developing a detailed understanding of the genes, proteins and metabolites involved in this valuable adaptation. Comprehensive knowledge of the CAM 'parts-list' will permit forward engineering of CAM into C3 crops. In particular, we are collaborating with US scientists on a plant synthetic biology project that aims to introduce CAM into poplar trees (http://cambiodesign.org). \nWe have performed whole genome and transcriptome sequencing (RNA-seq) for our model CAM systems, Kalanchoë fedtschenkoi and K. laxiflora, including detailed analysis of patterns of gene transcript abundance in CAM and C3 tissues of both species. This gene discovery work has identified candidate genes for engineering CAM into C3 crops. In order to refine the list of candidate CAM genes, recent work in the Hartwell lab has focused on silencing each candidate CAM gene in transgenic lines of Kalanchoë in order to refine the minimal parts-list for efficient CAM (e.g. Dever et al., 2015 Plant Physiology; Boxall et al., 2017 The Plant Cell).\nIn addition, we have recently used detailed gas exchange analysis to identify both weak and strong CAM species belonging to the genus Kalanchoë. The weak CAM species, K. gracilipes, is relatively thin leaved and uses C3 photosynthesis when well-watered, but weak CAM when drought stressed. It occupies a basal/ ancestral position in the Kalanchoë molecular phylogeny. By contrast, strong CAM species of Kalanchoë, such as K. hildebrandtii, are highly succulent and rely on CAM under all environmental conditions. These strong CAM species are also the most derived in evolutionary terms. These discoveries set the stage for comparative genomic analysis of CAM evolution in Kalanchoë, which will allow us to identify the genetic and epigenetic changes that are associated with the transition from weak and inducible CAM through to strong CAM in this diverse genus.\nThis project will focus on comparative analysis of our existing K. fedtschenkoi and K. laxiflora genomes and RNA-seq data with the newly decoded genomes and transcriptomes of K. gracilipes and K. hildebrandtii, which will be sequenced as part of our ongoing research in the coming months funded by our US Dept. of Energy "CAM biodesign" project. The sequences will be available prior to the start of this proposed PhD project. In the long term, this work will make a substantial contribution to the development of more drought tolerant, water use efficient bioenergy crops and new biofuel feedstock crops suitable for desert cultivation.\n\n"], "extra_text": ["", "\n\n\n\n"], "status": ["", "Active"]}
April 11, 2022, 1:48 a.m. Added 35 {"external_links": [21707]}
April 11, 2022, 1:48 a.m. Created 35 [{"model": "core.project", "pk": 5832, "fields": {"owner": null, "is_locked": false, "coped_id": "d3e40f7a-f383-466c-82fd-8e925825662d", "title": "", "description": "", "extra_text": "", "status": "", "start": null, "end": null, "raw_data": 27314, "created": "2022-04-11T01:41:49.610Z", "modified": "2022-04-11T01:41:49.610Z", "external_links": []}}]