Regulation of polyphenolics and genetic manipulation of lignins in grasses

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Title
Regulation of polyphenolics and genetic manipulation of lignins in grasses

CoPED ID
2a1b6766-5a12-4a8f-a50e-e650dd396080

Status
Closed

Funder

Value
£707,200

Start Date
March 31, 2008

End Date
March 30, 2012

Description

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For fermentation, pyrolysis based transport fuel applications, and possibly gasification, a reduction in lignin levels or altered lignin structures and distributions within the plant will enhance the value of grasses entering industrial processes; whereas for combustion applications, increased lignin levels and high calorific value would be desirable. We have established technologies for monitoring lignin and phenolic acids in natural, mutant and transgenic populations of forages and have been extending this to energy crops including Miscanthus. Brown midrib (bmr) mutants of maize are known to have modified lignin structure and exhibit increased digestibility and we have shown that these mutants show higher rates of enzyme mediated sugar release than control plants. As such it may be predicted that the lignocellulose fraction of bmr mutants of Miscanthus may be more amenable to both enzymic release of cell wall sugars by fermentation or for production of high quality bio-oils via pyrolysis. Characterised bmr mutants occur in the CAD and OMT genes of the lignin biosynthetic pathway and opportunities exist for generating bmr mutants in Miscanthus by down-regulation of CAD and OMT gene expression. The CAD gene has been cloned in Miscanthus and is being used in an RNAi experiment in Miscanthus to mis-express the gene.

Iain Donnison PI_PER
Gordon Allison COI_PER
Maurice Bosch COI_PER

Subjects by relevance
  1. Lignin
  2. Genes
  3. Biotechnology
  4. Energy crops
  5. Mutants
  6. Gene technology
  7. Fermentation industry
  8. Fermentation (alteration)
  9. Maize
  10. Computer-aided design

Extracted key phrases
  1. Lignin level
  2. Lignin structure
  3. Lignin biosynthetic pathway
  4. Characterised bmr mutant
  5. Regulation
  6. OMT gene expression
  7. High calorific value
  8. Transport fuel application
  9. High quality bio
  10. Miscanthus
  11. Genetic manipulation
  12. Cad gene
  13. Combustion application
  14. High rate
  15. Cell wall sugar

Related Pages

UKRI project entry

UK Project Locations