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[{"model": "core.projectfund", "pk": 19385, "fields": {"project": 4470, "organisation": 7, "amount": 350683, "start_date": "2015-11-01", "end_date": "2018-10-31", "raw_data": 20707}}]
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[{"model": "core.projectorganisation", "pk": 73799, "fields": {"project": 4470, "organisation": 3611, "role": "COLLAB_ORG"}}]
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[{"model": "core.projectorganisation", "pk": 73798, "fields": {"project": 4470, "organisation": 1667, "role": "COLLAB_ORG"}}]
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[{"model": "core.projectorganisation", "pk": 73797, "fields": {"project": 4470, "organisation": 5867, "role": "COLLAB_ORG"}}]
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[{"model": "core.projectorganisation", "pk": 73796, "fields": {"project": 4470, "organisation": 5849, "role": "COLLAB_ORG"}}]
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[{"model": "core.projectorganisation", "pk": 73795, "fields": {"project": 4470, "organisation": 5850, "role": "COLLAB_ORG"}}]
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[{"model": "core.projectorganisation", "pk": 73794, "fields": {"project": 4470, "organisation": 1380, "role": "COLLAB_ORG"}}]
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[{"model": "core.projectorganisation", "pk": 73793, "fields": {"project": 4470, "organisation": 5851, "role": "COLLAB_ORG"}}]
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[{"model": "core.projectorganisation", "pk": 73792, "fields": {"project": 4470, "organisation": 5765, "role": "COLLAB_ORG"}}]
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[{"model": "core.projectorganisation", "pk": 73791, "fields": {"project": 4470, "organisation": 5852, "role": "COLLAB_ORG"}}]
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[{"model": "core.projectorganisation", "pk": 73790, "fields": {"project": 4470, "organisation": 5853, "role": "COLLAB_ORG"}}]
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[{"model": "core.projectorganisation", "pk": 73789, "fields": {"project": 4470, "organisation": 5855, "role": "COLLAB_ORG"}}]
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[{"model": "core.projectorganisation", "pk": 73788, "fields": {"project": 4470, "organisation": 5856, "role": "COLLAB_ORG"}}]
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[{"model": "core.projectorganisation", "pk": 73787, "fields": {"project": 4470, "organisation": 5857, "role": "COLLAB_ORG"}}]
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[{"model": "core.projectorganisation", "pk": 73786, "fields": {"project": 4470, "organisation": 5490, "role": "COLLAB_ORG"}}]
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[{"model": "core.projectorganisation", "pk": 73785, "fields": {"project": 4470, "organisation": 5858, "role": "COLLAB_ORG"}}]
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[{"model": "core.projectorganisation", "pk": 73784, "fields": {"project": 4470, "organisation": 5859, "role": "COLLAB_ORG"}}]
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[{"model": "core.projectorganisation", "pk": 73783, "fields": {"project": 4470, "organisation": 5860, "role": "COLLAB_ORG"}}]
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[{"model": "core.projectorganisation", "pk": 73782, "fields": {"project": 4470, "organisation": 2175, "role": "COLLAB_ORG"}}]
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[{"model": "core.projectorganisation", "pk": 73781, "fields": {"project": 4470, "organisation": 1564, "role": "COLLAB_ORG"}}]
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[{"model": "core.projectorganisation", "pk": 73780, "fields": {"project": 4470, "organisation": 2524, "role": "COLLAB_ORG"}}]
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[{"model": "core.projectorganisation", "pk": 73779, "fields": {"project": 4470, "organisation": 5854, "role": "COLLAB_ORG"}}]
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[{"model": "core.projectorganisation", "pk": 73778, "fields": {"project": 4470, "organisation": 467, "role": "LEAD_ORG"}}]
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[{"model": "core.projectperson", "pk": 45368, "fields": {"project": 4470, "person": 6423, "role": "COI_PER"}}]
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[{"model": "core.projectperson", "pk": 45367, "fields": {"project": 4470, "person": 6424, "role": "PI_PER"}}]
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{"title": ["", "14 ERA-CAPS Mechanistic Analysis of Quantitative Disease Resistance in Brassica by Associative Transcriptomics"], "description": ["", "\nBrassica napus, a major world-wide crop, comprises a range of crop types including oilseed rape (OSR), grown for edible and industrial oil, biodiesel, protein for animal feed as well as leaf and root vegetables. Diseases are a major factor limiting production, a threat increasing due to climate change and the imminent withdrawal of agrochemicals in Europe. Improved disease control is an urgent priority and breeders are increasingly using quantitative disease resistance (QDR), which is considered broad-spectrum and durable.\n\nThis research will identify the most useful QDR genes for OSR breeding and understand the mechanisms behind this to enable predictions of their effectiveness and durability. Our consortium combines the leading expertise on the major OSR pathogens, the latest research on defence mechanisms of resistance and expertise in association genetics to identify effective QDR genes. Our industrial partner, KWS, will provide expertise on deployment of QDR in the field and on the development of genetic markers for molecular breeding of improved OSR varieties.\n\nWe will identify resistance to the most important pathogens of OSR: Sclerotinia sclerotiorum, Verticillium spp, Leptosphaeria maculans, Alternaria brassicicola, Pyrenopeziza brassicae, and the model pathogens Pseudomonas syringae and Botrytis cinerea. A panel of 192 diverse B. napus lines will be screened for resistance against these pathogens in controlled environments and at KWS field trial sites. Schools will contribute in a 'citizen science' project and evaluate resistance at locations throughout Europe. In the same lines, we will quantify induced defence responses to conserved pathogen-associated molecular patterns (PAMPs). We will also quantify salicylic acid, lignin, phenylpropanoid, glucosinolate, and indole metabolites that are implicated in resistance mechanisms. Using association transcriptomics, we will identify resistance gene loci against multiple pathogens and understand how this relates to metabolite production and PAMP-triggered immunity.\n\nTo test hypotheses about their contribution to resistance, we include studies on specific genes. Whilst glucosinolates contribute to resistance they can reduce the quality of seed. GTR1 and GTR2 are transporters in Arabidopsis that control the allocation of glucosinolates to seeds. We will test gtr1 gtr2 mutants for fitness and create gtr TILLING mutants in Brassica rapa (B. napus A genome) to measure the glucosinolate partitioning between leaves and seed. The work could enable development of OSR with high leaf glucosinolate content for resistance, without compromising seed quality. We will introduce tomato receptor Ve1 into B. napus and assess its ability to mediate resistance against Verticillium wilt. \n\nThis research will lead to more sustainable production of OSR, with higher productivity through lower vulnerability to biotic stress and less reliance on chemical inputs.\n\n"], "extra_text": ["", "\nTechnical Abstract:\nOilseed rape (OSR, Brassica napus) is a major crop worldwide, producing edible oil, biodiesel and protein for animal feed. Diseases are a major factor limiting OSR production and improved control is an urgent priority. Breeders are increasingly using quantitative disease resistance (QDR) which is considered broad-spectrum and durable. This proposal addresses the current gap in our knowledge which is the identification of the most useful QDR for breeding. \n\nThe first layer of active defence in plants is based on the perception of pathogen (or microbe) associated molecular patterns (PAMPs/MAMPs) leading to PAMP-triggered immunity (PTI). PAMPs are essential molecules, conserved in entire kingdoms of microbes, and are recognised by pattern recognition receptors (PRRs) in plants. Within ERA-PG (PRR-CROP) we developed methods for studying PTI in Brassica crops. \n\nWe will identify gene loci contributing to QDR against the most important pathogens of OSR using the novel method of associative transcriptomics (AT), developed at JIC in B. napus by the Bancroft group. Using a 'B. napus diversity panel' of 192 diverse lines we will quantify resistance to the most important pathogens: Sclerotinia sclerotiorum, Verticillium spp, Leptosphaeria maculans, Alternaria brassicicola, Pyrenopeziza brassicae, Pseudomonas syringae and Botrytis cinerea. We will quantify induced defence responses to PAMPs and measure salicylic acid, lignin, phenylpropanoid, glucosinolate, and indole metabolites that are implicated in resistance mechanisms. Using AT, we will identify resistance gene loci against multiple pathogens and understand how this relates to metabolite production and PAMP-triggered immunity. We will also investigate glucosinolate partitioning between leaves and seed using mutants of GTR1 and GTR2 transporters and introduce tomato receptor Ve1 into B. napus and assess its ability to mediate resistance against Verticillium wilt.\n\nPotential Impact:\nPathways to Impact: Our impact plan for non-academic users and beneficiaries is designed primarily to target those communities that are either most affected by the disease problems or involved in formulating policy relevant to disease management. Light leaf spot, caused by the fungal pathogen Pyrenopeziza brassicae, and phoma stem canker, caused by Leptosphaeria spp., are damaging diseases on oilseed rape in the UK, causing substantial yield losses. The information gained from the project will help agronomists to make recommendations to growers on choice of cultivars. New knowledge from this project will help breeders avoid breakdown of cultivar resistance. It will also help improve disease resistance rating for the HGCA Recommended List (RL). Results of this project will be communicated to policy-makers to guide forward planning as part of strategies to achieve the government climate change mitigation target for UK agriculture (Food 2030) by reducing greenhouse gas emissions through improved disease control. The project consortium members have a good track record for engaging with all of these communities.\n\nScientific Impact: Advancing fundamental scientific knowledge. This project will generate fundamental knowledge on ill-defined pathosystems that are of major significance to oilseed rape production in the UK. The genome-enabled technologies are not only expected to ameliorate the impact of light leaf spot and phoma stem canker disease pressure in the UK, but also they will be applicable to closely related disease problems like barley leaf spot caused by Rhynchosporium commune. The techniques developed in this study are expected have a significant impact on UK and international agricultural industries. Results from the project will reach the scientific community, including plant pathologists, agronomists and crop scientists through publications in prestigious peer-reviewed international journals The principal investigators will actively promote the research at international conferences. New findings will also be communicated as topic sheets or posters at agricultural events (e.g. Cereals', field days, road-shows, workshops). Details of the project will be made available through the research pages of consortium members.\n\nEconomic Impact: New breeding lines and disease management. UK agriculture will benefit from the scientific knowledge gained through this project. The breeding company involved in the project will develop breeding lines and commercialize cultivars with stronger and more durable resistance. Through participation of industry in the consortium, needs of end-users are better addressed. New knowledge about light leaf spot and phoma stem canker control will be disseminated to the industry primarily through on-farm advisory and knowledge transfer teams and breeder commercial marketing teams, and more widely through inter-linked websites.\n\nSocial Impact: Improvement of food security. The proposed study will have a large impact on the treatment of crop diseases due to generation of more resistant breeding lines and cultivars. This will positively affect the UK population but also have implications for the rest of Europe and beyond. Using the results of the project, we will increase the awareness of the problems associated with crop diseases and subsequent food security. This information will be disseminated via press releases into the popular scientific press and through publically accessible websites.\n\nExploitation and Application. In order to maximise impact, findings need to be translated into practical solutions and placed in the public domain as quickly as possible. The communication and engagement mechanisms described have been developed specifically for this purpose. The involvement of a wide range of partners (scientists, breeders, advisors) will ensure that results are exploited directly in the arable sector of the agricultural industry during the course of the project.\n\n\n"], "status": ["", "Closed"]}
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{"external_links": [16731]}
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[{"model": "core.project", "pk": 4470, "fields": {"owner": null, "is_locked": false, "coped_id": "259d8989-49cb-46e5-954e-df8bb4ed5594", "title": "", "description": "", "extra_text": "", "status": "", "start": null, "end": null, "raw_data": 20693, "created": "2022-04-11T01:38:39.489Z", "modified": "2022-04-11T01:38:39.489Z", "external_links": []}}]
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