Understanding the behavioural properties of single molecules or larger scale populations interacting with single molecules is currently a hotly pursued topic in nanotechnology. This arises from the potential such techniques have in relation to applications such as targeted drug delivery, early-stage detection of disease, and drug screening. Although label and label-free single-molecule detection strategies have existed for a number of years, currently lacking are efficient methods for the controllable delivery of single molecules in live cells. In this project we asymmetric voltage pulses will be used for label-free detection and delivery of single molecules in live cells through the single nanopores with "on-demand" timing resolution. We will develop controllable delivery of single DNA molecules with simultaneous label-free detection. Such the technology can enable that even highly diluted unamplified molecular populations can be efficiently delivered in single cells with control of the time of delivery and the number of molecules delivered. The application of this technology in vitro can open the door to using nanopores as a single molecule delivery tool, which is expected to have a broad range of applications, including targeted delivery of nucleic acids, gene regulation, infection, and single-molecule PCR.