History of changes to: Human organoid modelling of neurogenetic epilepsy syndromes
Date Action Change(s) User
Nov. 27, 2023, 2:12 p.m. Added 35 {"external_links": []}
Nov. 20, 2023, 2:03 p.m. Added 35 {"external_links": []}
Nov. 13, 2023, 1:33 p.m. Added 35 {"external_links": []}
Nov. 6, 2023, 1:31 p.m. Added 35 {"external_links": []}
Aug. 14, 2023, 1:30 p.m. Added 35 {"external_links": []}
Aug. 7, 2023, 1:31 p.m. Added 35 {"external_links": []}
July 31, 2023, 1:34 p.m. Added 35 {"external_links": []}
July 24, 2023, 1:35 p.m. Added 35 {"external_links": []}
July 17, 2023, 1:34 p.m. Added 35 {"external_links": []}
July 10, 2023, 1:26 p.m. Added 35 {"external_links": []}
July 3, 2023, 1:26 p.m. Added 35 {"external_links": []}
June 26, 2023, 1:25 p.m. Added 35 {"external_links": []}
June 19, 2023, 1:27 p.m. Added 35 {"external_links": []}
June 12, 2023, 1:29 p.m. Added 35 {"external_links": []}
June 5, 2023, 1:33 p.m. Added 35 {"external_links": []}
May 29, 2023, 1:27 p.m. Added 35 {"external_links": []}
May 22, 2023, 1:28 p.m. Added 35 {"external_links": []}
May 15, 2023, 1:31 p.m. Added 35 {"external_links": []}
May 8, 2023, 1:37 p.m. Added 35 {"external_links": []}
May 1, 2023, 1:27 p.m. Added 35 {"external_links": []}
April 24, 2023, 1:34 p.m. Added 35 {"external_links": []}
April 17, 2023, 1:28 p.m. Added 35 {"external_links": []}
April 10, 2023, 1:24 p.m. Added 35 {"external_links": []}
April 3, 2023, 1:26 p.m. Added 35 {"external_links": []}
Jan. 28, 2023, 11:08 a.m. Created 43 [{"model": "core.projectfund", "pk": 26916, "fields": {"project": 4109, "organisation": 49, "amount": 0, "start_date": "2021-09-30", "end_date": "2024-09-29", "raw_data": 42709}}]
Jan. 28, 2023, 10:52 a.m. Added 35 {"external_links": []}
April 11, 2022, 3:46 a.m. Created 43 [{"model": "core.projectfund", "pk": 19024, "fields": {"project": 4109, "organisation": 49, "amount": 0, "start_date": "2021-09-30", "end_date": "2024-09-29", "raw_data": 19239}}]
April 11, 2022, 3:46 a.m. Created 41 [{"model": "core.projectorganisation", "pk": 72639, "fields": {"project": 4109, "organisation": 1377, "role": "LEAD_ORG"}}]
April 11, 2022, 3:46 a.m. Created 40 [{"model": "core.projectperson", "pk": 44670, "fields": {"project": 4109, "person": 803, "role": "STUDENT_PER"}}]
April 11, 2022, 3:46 a.m. Created 40 [{"model": "core.projectperson", "pk": 44669, "fields": {"project": 4109, "person": 5999, "role": "SUPER_PER"}}]
April 11, 2022, 1:47 a.m. Updated 35 {"title": ["", "Human organoid modelling of neurogenetic epilepsy syndromes"], "description": ["", "\nRare single gene disorders manifest in the neonatal period. The Next Generation Children's Project has used whole genome sequencing of patients and their parents to diagnose 50 patients with neurogenetic epilepsy syndromes. For example, Kv7.2 voltage-gated potassium channel subunit dysfunction is associated with KCNQ2 mutations, with resulting increased network excitability. It is increasingly clear that epilepsy-causing gene mutations affect the major glial cell populations, principally astrocytes, rather than neurons. To date, human induced pluripotent stem cell-derived (iPSC) neurons have been generated with epilepsy-causing potassium/sodium channel mutations and gene deletions, however, lack the ability to establish human cortical connections. The development of human three-dimensional cerebral organoid models (CO) that incorporate circuit forming neuron and glial phenotypes present a unique opportunity to develop new approaches in precision medicine for paediatric epilepsy patients. This project aims to generate CO from CRISPR/Cas9 KCNQ2 and KCNJ10-edited H9 ECS lines and from patient-derived iPSC-line. To assess if stem cell-derived CO harbouring KCNQ2 or KCNJ10 mutations show altered cortical plate development, assessing CO cell composition by immunolabeling for cell type-specific markers (GAD2/ CTIP2+). To show whether CO slices with perturbed KCNQ2 and KCNJ10 function or expression show bursts in activity and whether this can be suppressed by Na+ or K+ channel drugs.\n\n"], "extra_text": ["", "\n\n\n\n"], "status": ["", "Active"]}
April 11, 2022, 1:47 a.m. Added 35 {"external_links": [15670]}
April 11, 2022, 1:47 a.m. Created 35 [{"model": "core.project", "pk": 4109, "fields": {"owner": null, "is_locked": false, "coped_id": "7e49f24d-0309-4a2d-8807-498be9396bb6", "title": "", "description": "", "extra_text": "", "status": "", "start": null, "end": null, "raw_data": 19223, "created": "2022-04-11T01:37:56.734Z", "modified": "2022-04-11T01:37:56.734Z", "external_links": []}}]