Molecular mechanisms of toxicity from exposure to biofuel emissions

d255b889-2bee-4bd1-975e-63bd4a341fac

Closed

MRC
MRC
MRC
MRC
MRC

£2,500,000

Feb. 1, 2018

Sept. 30, 2021

Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.

Technical Abstract:
In response to concerns over fossil fuel consumption, combustion of biomass and biofuel alternatives has been encouraged in Europe. While cleaner than fossil diesel emissions in terms of sulphur, CO and hydrocarbons, biodiesel emissions are rich in ultrafine particles and NOx and targeted studies indicate that they induce stronger inflammation and oxidative stress.

Early omics screens demonstrated that the molecular interactions that drive pulmonary responses to biodiesel emissions differ from those caused by fossil-diesel exhaust. Combining mRNA and miRNA sequencing with epigenomic arrays, my research aims to characterise transcriptional dysregulation in the lungs of individuals who have been exposed to biodiesel emissions under controlled chamber conditions. This work is accompanied by a multi-platform metabonomic urinalysis, which explores non-invasively accessible signatures of susceptibility to biodiesel emission toxicity.

Relatively little is known of wood smoke toxicity, but exposure has been shown to induce inflammation, cell death and genotoxicity in airway models. Mirroring my biodiesel study, I aim to characterise transcriptional dysregulation in the pulmonary tissue and activated inflammatory cells of exposed volunteers.